Journal: Life
Article Title: Successful Production of Offspring Derived from Phospholipase C Zeta-Deficient Sperm by Additional Artificial Activation
doi: 10.3390/life13040980
Figure Lengend Snippet: Phenotype of the Plcz1 − / − and Plcz1 − / − eCS − / − mice. ( A ) Histology of the Plcz1 − / − and Plcz1 − / − eCS − / − muse testes. Testes were collected from wildtype (WT) ( a , a’ ), Plcz1 − / − ( b , b’ ), and Plcz1 − / − eCS − / − mice ( c , c’ ). Paraffin sections (6 µm) were stained with H&E. Scale bars: 1 mm ( a – c ) and 100 µm ( a’ – c’ ). ( B ) Motility of Plcz1 − / − and Plcz1 − / − eCS − / − mouse spermatozoa. Sperm motility was analyzed by the SMAS. p > 0.05. ( C ) Concentration of Plcz1 − / − and Plcz1 − / − eCS − / − mouse spermatozoa. Sperm motility was analyzed by the SMAS. p > 0.05.
Article Snippet: Sperm motility was analyzed using the SMAS sperm motility analysis system (DITECT Corporation, Tokyo, Japan).
Techniques: Staining, Concentration Assay